INTERNATIONAL CONFERENCE

ON

MOLECULAR & CELLULAR 

ELECTRON TOMOGRAPHY

and

2^nd WORKSHOP

on

ELECTRON TOMOGRAPHY OF BIOLOGICAL SPECIMENS

NEW DELHI, INDIA

Workshop:   17-20, April 2023
Conference: 21-22, April 2023

Electron Microscope Facility, Department of Anatomy,

All India Institute of Medical Sciences (AIIMS), New Delhi

THEME OF THE CONFERENCE

vRecent advances in Electron Tomography in biological & biomedical field

vIn-situ architecture of the macromolecular assembly in cells

vLatest developments in sub-tomogram classification and averaging

vCryo-ET in disease diagnostics & healthcare

HIGHLIGHTS OF WORKSHOP

vSample Preparation (Plunge freezing, High-Pressure Freezing, CEMOVIS etc.

vScreening and Tilt-Series acquisition on 200/300KV CryoTEM

vData processing with IMOD , eTOMO and other software

vSub tomogram averaging, reconstruction and segmentation

Overview of the International Conference: Electron Tomography (ET) with 3D image reconstruction (RT & Cryo) showed the potential to revolutionize biology and life science as a powerful tool for in situ macromolecular structure determination from their projections recorded at several angles. This can investigate the macromolecular complexes in the context of the cell or tissue in native environments with atomic-scale resolution in their spatial relationships and interactions with cells or tissues. Cryo-ET is now rapidly developing a diagnostic and research tool that enables structural biologists to determine the structure of proteins in their native cellular environment to sub-nanometer resolution. The upcoming two days of International conferences on Molecular and Cellular Electron Tomography (MCET-2023) will explore the potential of cryo-ET in the biomedical, biological, and diagnostics fields. 

Overview of Workshop (Electron Tomography of Biological Specimen): We are organizing a four-day workshop and training program that will focus on providing introductory knowledge of Electron Tomography (RT and Cryo), hands-on for sample preparation using plunge freezing, cryo-ultramicrotomy, or cryo-focused ion- beam, glow discharge, growing cells on grids, transfer of grid to the EM, and handling, tilt series data collection, and processing with IMOD and eTomo software. The workshop will be planned with theoretical/methodological lectures and hands-on practical sessions simultaneously. This training is scheduled for capacity-building and improvement of research for the Indian researchers, post-docs, faculties, scientists, and R&D industries working in the relevant area.

About EMF: The Electron Microscopy Facility (EMF) was established in the year 1971 in the Department of Anatomy, All India Institute of Medical Science (AIIMS), serving as a nodal center for excellence in biological electron microscopy for disease diagnostics/ ultrastructural imaging to help more than 1900 research laboratories (registered with EMF) across India to foster their research for electron microscopy imaging. We are regularly providing training for scientific investigators and technicians for biological electron microscopy since 1986.

Selection Criteria for Workshop: This workshop accommodates a maximum of 12-15 participants. Participants require to submit a small write-up explaining the statement of purpose regarding the importance of electron tomography in their research. The applicants must substantiate the relevance of attending the training program to their current research project or interest.

Selection Criteria for Conference: The conference is open to all with an elementary knowledge of biological electron microscopy, SPA, or Electron Tomography (RT/cryo-ET).

Registration process for Workshop and Conference:

The complete application form must contain the following details:

(1) Name (2) Age (3) Department and University/Institute Postal address (4) e-mail ID (5) mobile no. (6) Educational qualifications from graduation onward indicating a year of passing (7) Present affiliation & research experience in brief

(8) letter of recommendation from the present employer/research supervisor (for Ph.D. candidates) (9) write-up of present work/research interest including a statement of purpose regarding the importance of electron tomography in your research.

Point number 08 and 09 is not required for conference participants.

The complete application form may be sent to emfdbtsahaj@gmail.com or directly apply through the google form at this link: 

https://docs.google.com/forms/d/e/1FAIpQLSerhrTHT4k3JopxiaWLbNvR6-GQc_f2wqi2h-rIDy18sp6PFQ/viewform?usp=sf_link

or available on http://saifaiims.com before Feb 28th, 2023.

Only, the finally selected candidates will be informed by email on 10th March 2023, and they must deposit/transfer the non-refundable registration fee before  31st March 2023.

Registration Fee:

(Registration fee includes work­shop fee, work­shop mate­ri­als, cater­ing dur­ing the day includ­ing lunch)

NOTE: The course fee does not include boarding. Participants are advised to arrange for boarding well ahead of the conference.

Organised By:

Electron Microscope Facility, Department of Anatomy, 

All India Institute of Medical Sciences (AIIMS), New Delhi

Tel: +91-011-26593568; 26549121 & 27; emfdbtsahaj@gmail.com

Organising Committee:

Dr. Subhash C Yadav 

Dr. Ravi Prakash

Dr. Prabhakar Singh

Prof. T. C. Nag

Advisory Committee:

Dr. Garima Gupta, Department of Biotechnology, Govt. of India

Prof A. Shariff, Head of the Department, Anatomy, AIIMS, New Delhi

Prof. Manidipa Banerjee, Kusuma School of Biological Sciences, IIT, Delhi

Prof. Suneel Kateriya, School of Biotechnology, JNU, New Delhi

Dr. Gopaljee Jha, NIPGR, New Delhi

Dr. C. V. Srikanth, RCB, Faridabad

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