National Workshop Electron Tomography of Biological Specimens
Electron Microscope Facility at AIIMS-New Delhi is organizing a five-day workshop/training program on
“Electron Tomography of Biological Specimens”
(May 9-13, 2022)
for the Ph.D., post-docs, and
faculties of Indian Institutions, Universities and Industries.
This training program
introduces the principles, technique, instrumentation, and application of
Electron Tomography in 3D ultra structural imaging at RT and cryo condition for
biological samples. This training program provides the basics and hands-on
training in sample preparation for electron tomography, tilt series data
collection, tomogram generation, sub tomogram averaging, segmentation, and
model building.
The workshop covers
theoretical /methodological lectures and practical sessions with hands-on
training on specimen preparation for Electron tomography such as glow
discharge, growing cell on grid, plunge freezing, cryo ultramicrotomy, transfer
grid to the electron microscope, microscope handling, tilt series, data
collection, and data processing with software IMOD and eTomo.
This training is scheduled for the capacity-building under the DST STUTI, DST SAIF and DBT SAHAJ programs for the Indian national researchers, post-docs, faculties, scientists and R&D industries working in the relevant area. This training would be beneficial to improve their research quality in the respective area. A one-page write-up explaining the statement of purpose regarding the importance of electron tomography in their research would be essential. The applicants must substantiate the relevance of attending the training program to their current research project or interest.
Electron Tomography (ET) in conjugation with advanced 3D image reconstruction techniques has revolutionized biology and life science and emerged as a powerful tool for in situ macromolecular structure determination from their projections recorded at several angles. This can investigate the macromolecular complexes in the context of the cell or tissue in native environments with atomic-scale resolution in their spatial relationships and interactions with cells or tissues. Cryo-ET is now rapidly developing a diagnostic and research tool that enables structural biologists to determine the structure of proteins in their native cellular environment to sub-nanometer resolution. In a cryo-ET, a biological specimen is flash-frozen (preserves the sample in a hydrated, close-to-native state), thinned to an appropriate thickness, and multiple images are captured along with the tilt axis. The images are aligned and merged using computational techniques to reconstruct a three-dimensional picture or tomogram or by sub-tomogram averaging.
We are organizing a five-day workshop and training program (offline) that will focus on providing introductory knowledge of Electron Tomography (RT and cryo), hands-on for sample preparation using plunge freezing, cryo-ultramicrotomy, or cryo focused-ion-beam, glow discharge, growing cells on grids, transfer of grid to the EM, and handling, tilt series data collection, and processing with IMOD and eTomo software. The workshop will be planned with theoretical/methodological lectures and hands-on practical sessions simultaneously.
Selection Criteria:
This
workshop accommodates a maximum of 15 participants. Participants required to
submit a small write-up explaining the statement of purpose regarding the
importance of electron tomography in their research would be essential. The
applicants must substantiate the relevance of attending the training
program to their current research project or interest.
Registration:
The
complete application form must contain the following details- (1) Name (2) Age (3)
Department and University/Institute Postal address (4) e-mail ID (5) mobile no.
(6) Educational qualifications from graduation onward indicating a year of
passing and marks obtained (7) Present affiliation & research experience in
brief (8) letter of recommendation from the present employer/research
supervisor (for Ph.D. candidates) (9) write-up of present work/research
interest including a statement of purpose regarding the
importance of electron tomography in your research.
The complete application form forwarded
through proper channels may be sent to emfdbtsahaj@gmail.com before April
10th, 2022.
Only, the finally selected candidates will be informed by email on 12 April 2022, and they must deposit/transfer the nonrefundable registration fee before 20 April 2022.
Registration Fee:
Rs 7500/- (For
Government Institution/University
Rs 15000/- (For
Private Institution/University, and Industries)
(Registration
fee includes workshop fee, workshop materials, catering during the
day including lunch for all five days)
NOTE: The course fee
does not include boarding. Participants are advised to arrange
for boarding well ahead of the training.
Organising Committee:
|
Dr. Ravi Prakash Organizing Secretary |
Dr. S.C. Yadav Course Organizer |
Prof. T. C. Nag Course Coordinator |
|
|
Electron Microscope Facility Department of Anatomy All India Institute of Medical Sciences (AIIMS) New Delhi Tel:
+91-011-26593568; 26549121; 26549127 |
|||
Advisory Committee:
Prof A. Shariff, Head of the Department,
Anatomy, AIIMS, New Delhi
Prof. Manidipa Banerjee, Kusuma School
of Biological Sciences, IIT, Delhi
Prof. Suneel Kateriya, School of
Biotechnology, JNU, New Delhi
Dr. Gopaljee Jha, NIPGR, New Delhi
Dr. C. V. Srikanth, RCB, Faridabad
Dr. Prabhakar Singh, EMF, Anatomy, AIIMS,
New Delhi
